Proteins are one of the most important macromolecules in the cell, carrying out a variety of functions including catalyzing biochemical reactions, serving as structural components of cells and tissues and regulating gene expression. Because of their complex properties and broad range of responsibilities, proteins need to be isolated from other cells and tissue components for accurate characterization and application. Our protein expression and purification services help you obtain pure samples for use in downstream experiments that require the highest quality materials (e.g. structural studies, enzyme assays or protein-protein interaction studies).
In order to obtain the highest quality recombinant proteins, our protein expression laboratories employ stringent screening and optimization processes. Parallel small-scale screening in bacterial, yeast, insect and mammalian cell cultures along with an innovative purify first platform enable us to find the best constructions for each target protein. This is followed by the recombinant expression of your target in large quantities using an automated instrument from Nuclera.
Top Protein Expression and Purification Services for Your Research
Viral vector-mediated transfection provides a solution to the challenge of delivering genes into hard-to-transfect cells for recombinant protein overexpression or knockdown applications. This technique utilizes adenoviruses, oncoviruses, or lentiviruses as delivery vehicles.
Affinity purification tags (e.g. GST, MBP) can be added during recombinant protein production to trap the resulting proteins for downstream analysis and remove impurities. Fluorescent labels can also be covalently coupled to the proteins during expression to facilitate imaging and interaction studies using biophysical techniques like optical tweezers. Molecular weight, purity and functionality of your recombinant protein can be further assessed with SDS-PAGE, Western blotting, mass spectrometry or isoelectric focusing.